A β₂-stimulating mAb did not increase adhesion of PMNs from the patient.

PMNs were pretreated with buffer (▪) or with the β₂-stimulating mAb KIM 185 (10 μg/mL) for 20 minutes at 37°C, and adhesion to immobilized gelatin was measured after a 40-minute incubation. In some replicates, a blocking mAb directed against β₂, 60.3 (10 μg/mL), was included to document that adhesion was dependent on β₂ integrins (“anti-β₂,” ░). The bars indicate the means and the error bars the ranges of the determinations (n = 2).