Fig. 5.
LADV cells express surface β1 and β2 integrins at levels similar to those on control lymphoblastoid cells.
LADV cells derived from the patient and age-matched control lymphoblastoid cell lines C1 and C2 were analyzed by flow cytometry as described in “Patient, materials, and methods.” Solid profiles indicate staining with the following mAbs: the β1-specific mAb UN29, the α4-specific mAb P4C2, the α5-specific mAb P1D6, the αL-specific mAb BCA1, the β2-specific mAb 60.3, and the CD45-specific mAb H130. Binding of the primary antibodies were detected with FITC-conjugated goat antimouse IgG secondary antibodies (heavy and light chain specific). Open profiles indicate fluorescence of cells incubated with the secondary antibody alone (anti-IgG2a,κ in panels showing staining with anti-β2or anti-αL and anti-IgG1a,κ in all others).