The LADV EBV-transformed lymphoblastoid cell line demonstrates impaired β₂ integrin–dependent adhesion.

LADV cells (░) or control lymphoblasts (2 different control lines, ▪ and ■) were incubated in wells coated with immobilized ICAM-1 for 15 minutes at 37°C. In parallel, the cells were treated with buffer, PMA (10⁻⁷ M), or Mn²⁺ using a cation substitution protocol as outlined in “Patient, materials, and methods.” In some wells, the blocking anti-β₂ mAb, 60.3 (10 μg/mL), was added to the incubation mixture. The bars indicate the means and the error bars the ranges of duplicate determinations.