Fig. 9.
The β1 and β2 proteins from LADV cells are of expected size.
(A) LADV cells and control lymphoblastoid cells were lysed and proteins in the lysates were immunoprecipitated with the anti-β2, mAb 60.3, or with an irrelevant control antibody, anti-CD14. The proteins were then separated on a 7.5% SDS-PAGE gel, transferred to a nitrocellulose membrane, and Western blot analysis was performed using the anti-β2 mAb 60.3. Equal amounts of protein were applied to each lane. The β2 protein band immunoprecipitated from LADV cells is of identical size and is at least as intense as that from control cells. (B) Transformed LADV cells, control lymphoblastoid cells, or Jurkat T cells were lysed, the proteins were separated on a 7.5% SDS-PAGE gel under nonreducing conditions, and analyzed by Western blot, using the anti-β1 mAb 13. Although there are variations in loading, the proteins from each cell type are of similar size.