Transduction of DCs with multiply attenuated lentiviral vector does not alter the allostimulatory capacity of these DCs.

Untransduced DCs (DC.utr) or transduced DCs (in this case with the HIG vector, DC.HIG) were cultured in graded doses with allogeneic (allo) or syngeneic (syn) T cells. Incorporation of ³H-TdR was measured after 6 days of coculture. PBMCs were used as a control to assess DC potency. X-axis: number of stimulating cells per 10⁵ T cells (logarithmic scale); y-axis: ³H-TdR incorporation by T cells (logarithmic scale).