Flu-specific CTL activation by DCs transduced with the lentiviral vector HFIG.

DCs were infected with HIG or HFIG or left uninfected. The ability of HFIG-transduced DCs to activate Flu-specific CTLs (DC.HFIG) was compared with untransduced or HIG-transduced DCs either nonpulsed (DC⁻, DC.HIG⁻) or pulsed (DC⁺, DC.HIG⁺) with the Flu peptide. CTL assays were performed after incubation of the DCs with autologous T cells for 8 to 10 days. T2 cells pulsed with the Flu peptide (T2⁺) or with the irrelevant MART-1 peptide (T2⁻) were used as targets. Data are shown for 1 of 3 independent experiments.