Fig. 2.
Activation of endothelial p38 by NiCl2 and TNFα.
HUVECs were stimulated with 1.5 mM NiCl2 (A-B) or 1.5 ng/mL TNFα (C-D) for the time intervals indicated. Relative p38 activity was quantified by densitometrical evaluation of autographs and is depicted as mean ± SEM of 3 independent experiments. Immune-complex kinase assays were performed as described in “Materials and methods” with the use of 3pK K73M as substrate. Protein loads were controlled by Western blot with the use of an antiserum against p38.
