Fig. 4.
Inhibition of NiCl2-induced endothelial p38 activation in a concentration-dependent manner by SB202190 but not D609.
To study the effects of pharmacological inhibitors SB202190 and D609 on p38 activity, HUVECs were left unstimulated or exposed to arsenite (A) or NiCl2 (B) for 60 minutes or to TNFα (C) for 30 minutes in the absence or presence of 1.25, 5.0, or 20 μM SB202190 or 1.56, 6.25, or 25 μg/mL D609, which was added to the culture medium 30 minutes prior to stimulation. After cell lysis, MAPKAP kinases 2 and 3 were immunoprecipitated and assayed for activity with Hsp27 used as substrate.
