Fig. 2.
Transcription of γ chain in transfected cells.
Total cellular RNA was isolated from cells cotransfected with FcαR and WT or altered γ-chain cDNA. The presence of γ-chain message was detected by RT-PCR using γ-chain–specific primers, as described in “Materials and methods.” The γ-chain–specific PCR product was detected as shown in lane 1 (γ-chain cDNA+ control), lane 4 (FcαR + WT γ chain), lane 5 (FcαR + IIA ITAM γ chain), and lane 6 (FcαR + Y → F γ chain). The γ-chain–specific product was not detected when RNA was not added (lane 2) or with parent IIA1.6 cells (lane 3).
