The effect of protease inhibitors on the production of antibacterial protegrins in wound fluid.

Wound fluids (100 μL) induced by HBSS with solvent only (S), with 0.25 mM N-methoxysuccinyl-AAPV chloromethyl ketone (NEI), or with 0.25 mM ZGLF chloromethyl ketone (CGI) were analyzed by (A) Coomassie-stained AU-PAGE, (B) Western blot with anti-PG mAb, and (C)E coli gel overlay. We used 1 μg PG-1 as a standard. We also used 1 μg proprotegrin (pPG) as a control in the Western blot (panel B, arrow).