Supplementing wound chambers in vivo with exogenous PG-1 restores the microbicidal activity of NEI-treated wound fluid.

(A) The complete clearance of S epidermidis from wound fluid, as measured by a CFU assay at 24 hours, was achieved by adding either 20 or 200 μg/mL PG-1 during wound chamber preparation. PG-1 (1 μg/mL) added 4 hours after chamber preparation normalized the activity of the NEI-treated fluid to that of the solvent-treated control (denoted by #), as determined by a CFU assay at 24 hours. (B) Wound fluids from panel A were subjected to RDAs against S epidermidis. The addition of 20 μg/mL PG-1 in vivo concurrently with NEI and bacteria (t = 0) or 1 μg/mL PG-1 at 4 hours restored the antimicrobial activity of NEI-treated wound fluid (n = 3).