Activated forms of FGFR3 identified in MM induce transformation in NIH 3T3 in a dose-dependent fashion.

(A) NIH 3T3 cells were transfected in triplicate with 200 ng empty vector (pCEFL) and with 50, 100, and 200 ng FGFR3 carrying the activating mutation K650E. Cellular foci were stained 12 days after transfection. (B) NIH 3T3 cells were transfected in triplicate with 0.1 μg (▪) and 1 μg () the indicated plasmids. Plates were scored for the presence of foci 12 to 14 days after transfection, after fixing and staining. We monitored the efficiency of transfection for each construct by counting the number of neomycin-resistant colonies on control plates.