Southern blot analysis.

Southern blot analysis shows no rearrangements of TCR genes in SNK-6 and rearrangements of the β-, γ-, and δ-chain genes in SNT-8. Panels show hybridization with the following probes: (A) the Cβ1 probe, (B) the Jγ1 probe, (C) the Jδ1 probe, (D) the Jδ3 probe. Human placenta DNA was used as a germline control. The letters above the pictures are BamHI (B), EcoRI (E),HindIII (H), and KpnI (K) digest. Note that the rearrangements of the γ-chain gene in SNT-8 cannot be detected by the BamHI, EcoRI, orHindIII digest. The lack of a 5-kb HindIII band hybridizing with the Jγ1 probe in SNT-8 is due to a polymorphism for a HindIII cleavage site.41