Fig. 5.
Human Lu gp transfectants specifically adhere to immobilized laminin 10/11.
Laminin preparations were titrated and tested for binding of fluorescently labeled K562 transfectants expressing human Lu gp in a microplate adhesion assay. The transfectants adhered to purified laminin 10/11 in a concentration-dependent manner (open circles, 55% binding at 1 × 10−12 mole added protein/well). The transfectants adhered only poorly to crude human, placental laminin 2/4 (closed triangles, 11% binding at 5 × 10−12 mole added protein/well) and to mouse laminin 1 (open squares, 3% binding at 5 × 10−12 mole added protein/well). Results are the mean percentage binding from 3 experiments ± 1 SD.
