PB CD34⁺ cells obtained from mobilized patients show reduced spontaneous and SDF-1–induced migration across fibronectin-coated filters compared to PB CD34⁺ cells of healthy mobilized donors.

Spontaneous and SDF-1–induced migration (100 ng/mL and 300 ng/mL) of PB-derived CD34⁺ cells after 4 hours of 6 healthy mobilized donors (■), 18 patients with nonhematologic malignancies (▨), and 10 patients with hematologic malignancies (▪). Spontaneous migration and SDF-1–induced migration (100 ng/mL and 300 ng/mL SDF-1) of PB CD34⁺ cells derived from healthy donors was significantly higher than from patients with nonhematologic or hematologic malignancies (* = P < .05; ** = P < .01; *** = P < .001). No significant differences in spontaneous and SDF-1–induced migration were observed between the 2 patient groups. Each bar represents the mean ± SEM.