Extracellular matrix degradation by RAW264.7 macrophages is inhibited by anti–annexin II IgG.

Cells (5 × 10⁵/well) were plated on insoluble [³H]-glucosamine–labeled human smooth muscle cell matrices in MSFM. Following adherence, media were replaced and 25 μg/mL monoclonal anti–annexin II (clone Z014, Zymed) or mouse IgG added. Cells were incubated with the antibodies (30 minutes, 37°C) prior to the addition of plasminogen (Plg, 1 μg/mL). Incubation media were recovered the next day and solubilized [³H]-activity determined as described in “Materials and methods.” The media and media plus plasminogen (Plg) controls depict the levels of [³H]-glucosamine released from insoluble extracellular matrix in the presence of plasminogen but in the absence of macrophages. Data represent the mean ± SEM of 4 replicate samples. nIgG indicates normal IgG.