Fig. 6.
Physical interaction between MITF and c-Jun.
(A) In vitro binding of c-Jun to MITF. 35S-labeled c-Jun was subjected to coprecipitation with GST-+-MITF, GST-mi-MITF, or GST-coated beads, and the protein complex was analyzed by SDS-PAGE. Coomassie brilliant blue staining for each GST fusion protein was also shown. (B) Coimmunoprecipitation of MITF and c-Jun. COS-7 cells were transfected with FLAG-tagged–c-Jun and Myc-tagged–+MITF or Myc-tagged–mi-MITF. Nuclear or cytoplasmic extract was subjected to immunoprecipitation with anti-Myc antibody. Precipitates were separated by SDS-PAGE and immunoblotted with anti-Myc or anti-FLAG antibody.
