Phenotype of CXCR3-expressing cells in human thymus.

Freshly isolated thymocyte suspensions were assessed for different surface markers by 4-color FACS analysis. (A) Thymocyte costaining for CXCR3 and CD3 showing 2 CD3⁺ subsets (R1, 1.3%; and R2, 0.4%) and one CD3⁻ subset (R3, 0.3%). (B-D) Costaining of the R1, R2, and R3 subsets for CD4 and CD8. (E-G) Costaining of the CXCR3⁺ subsets for TCRγδ and TCRαβ. (H-J) Staining of the CXCR3⁺ subsets for CD4 and CD56. Data shown are representative of 6 separate experiments.