Chemoattraction of thymocytes from human thymuses by I-TAC, IP-10, and Mig.

Freshly isolated thymocytes from thymuses of 5-day-old to 3-month-old children were assayed for chemotaxis in response to different concentrations of the 3 chemokines. (A) Values are expressed as percentage increase of cells migrated in response to I-TAC, IP-10, and Mig versus cells migrated in the presence of medium alone. Data represent mean values ± SE of 4 separate experiments. (B) Input and migrated thymocytes were costained for CD4 and CD8 and analyzed by FACSCalibur.