Effect of cytochalasin D and jasplakinolide on EC stiffening response induced by neutrophils.

Twenty-four-hour TNF-α–treated ECs were incubated with 1 μg/mL cytochalasin D or control vehicle (A) or with 5 μM jasplakinolide or control vehicle (B) for 30 minutes along with the anti–β1-integrin–coated beads. Cells were then washed twice, and the stiffness before or 2, 6, 10, and 15 minutes after neutrophil adherence was evaluated using magnetic twisting cytometry as described in “Materials and methods.” Data are expressed as mean ± SEM (n = 4). ●, control vehicle; ○, agonist. *P < .05 when compared to the vehicle-pretreated controls.