F-actin distribution in untreated or 24-hour TNF-α–activated ECs on neutrophil adherence.

F-actin was visualized using rhodamine-phalloidin stain as described. (A) Untreated ECs. (B) Untreated ECs with neutrophil adherence for 15 minutes. (C) Twenty-four-hour TNF-α–activated ECs. (D-F) Twenty-four-hour TNF-α–activated ECs with neutrophil adherence for 2, 6, and 10 minutes. Scale bars, 10 μm.