Northern blot analysis of Lp(a)-sensitive genes and genes not regulated by Lp(a).

Elutriation-purified monocytes from controls with low Lp(a) were incubated with purified LDL (100 μg/mL) and (60 μg/mL), purified Lp(a) (100 μg/mL) and (60 μg/mL) in 20% autologous serum or in 20% serum alone (control) for 18 hours. Total RNA was isolated and the expression of Lp(a)-sensitive genes (A) PAI-2, PAI-1, and genes not regulated by Lp(a) (B) MCP-1, adipophilin, IP-10, CD87, and GAPDH was analyzed.