DSBs of
TEL and AML1 genes as detected by LM-PCR. Representative results of LM-PCR are shown. PCR products were electrophoresed on 2% agarose gels and stained with ethidium bromide. (A) DSBs of TEL and AML1 genes in TS-2 and RS4;11 cell lines. Gene-specific primers used for seminested PCR are indicated in each panel. FBS(−) indicates cells treated with serum starvation; salicylic acid, cells treated with salicylic acid as described in “Materials and methods.” (B) Representative results of LM-PCR for BCR,β-globin, and GAPDH genes in TS-2 and RS4;11 cell lines. Primers used for LM-PCR are listed in Table 1. (C) DSBs ofTEL and AML1 genes in EB-LCLs after treatment with various chemical agents. Primers used for seminested PCR were indicated in parentheses. H2O represents negative control.
