Inverse PCR analysis of rearranged

TEL gene after serum starvation. (A) Representative results of inverse PCR-amplified fragments of the rearranged TEL gene after serum starvation. PCR products were electrophoresed on 0.8% agarose gels and stained with ethidium bromide. Control samples obtained before serum starvation (0 hour) show only normal products derived from the 4-kb HindIII fragment (arrow). Samples from 12 hours of serum starvation culture show rearranged products with smaller size than the germline band in RS4;11 and TS-2. (B) Nucleotide sequences of the breakpoints of theTEL gene and unknown sequences fused to DNA break ends in 5 rearranged clones.