Fig. 1.
Eosinophils express mRNA for SCF.
RNA was extracted from 5 × 106 fresh eosinophils (F. Eos) and eosinophils cultured overnight with enriched medium alone (Eos o.n.). As a positive control, total RNA from human foreskin fibroblasts (FSK) was used. As a negative control, a sample was obtained in the presence of water instead of reverse transcriptase (−RT). RNA was reverse-transcribed to cDNA and amplified with specific primers for SCF. The products were electrophoresed on a 1.8% agarose gel and then were stained with ethidium bromide. Molecular weight marker (D-15 marker NOVEX) was electrophoresed simultaneously with other PCR products (M).
