Eosinophils synthesize SCF.

Lysates were obtained from freshly isolated eosinophils (lane 2) and from eosinophils cultured in enriched medium for 4.5 hours (lane 3) and overnight (lane 4). Recombinant SCF was loaded as a positive control (lane 1). The protein extracts were analyzed on a 12.5% SDS-PAGE gel and blotted. The blot was probed with goat anti-human SCF antibodies followed by incubation with peroxidase-conjugated donkey anti-goat antibodies. The detection was performed by enhanced chemiluminescence.