Detection of the altered distribution of proviral MuLV during vincristine treatment.

(A) Schematic representation of the vectorette PCR strategy. The filled and open bars represent the MuLV proviral genome and mouse genomic DNA, respectively. The black, gray, and light gray arrows correspond to uvp, musp1, and musp2, respectively. The capital letters N, S and X correspond to restriction sites for NheI, SpeI, and XbaI, respectively. Further details are described in “Materials and methods.” (B) Vectorette PCR products separated by 10% acrylamide gel. The arrows indicate P-V–derivative specific bands; the sharp signs, P388- and P-V–derivative specific bands; and the asterisks, P388- and P-V–derivative non-specific bands.