Fig. 3.
Comparative analysis of gene expression in PB-derived MCs and CB-derived MCs.
CB-derived MCs and PB-derived MCs were primed with IL-4 for 6 hours and examined for gene expression screening using GeneChip. Average fluorescence differences of approximately 5600 genes (7129 probe-arrays) were scattered on the logarithmic graph according to the values obtained from PB-derived MCs (vertical) and CB-derived MCs (horizontal). Black crosses indicate transcripts judged to be present at least in either MC type, and gray crosses indicate transcripts judged to be absent or marginal in both MC types. FcεRIα mRNA (indicated with an arrow) was determined with Comparison Analysis software to be the fifth most increased transcript in PB-derived MCs. Similar results were obtained in another experiment using IL-4–unprimed MCs.