Examples of fusion genes detected by the multiplex RT-PCR.

Amplification products of 8 parallel multiplex RT-PCR reactions (left panels) and the corresponding split-out reactions (right panels). The product with the higher molecular weight represents the internal positive control, and the lower bands represent the specific fusion genes. (A) inv(16)(p13q22)—CBFB/MYH11 fusion gene. (B) t(15;17)(q21;q22)—PML/RARA. (Note: Due to the primer combinations for the detection of different breakpoints, a few of the translocations detected by the HemaVision kit can appear in more than one master reaction, as seen in lanes 4 and 8. In the right panel of B, split-out reactions correspond to master reaction no.8.) (C) t(8;21)(q22;q22)—AML1/MGT8 (M, 100-bp DNA Ladder (Promega).