OSM induction of NF-κB and AP-1 nuclear activity.

Nuclear extracts of SMCs were prepared following OSM (10 ng/mL) treatment. EMSA was performed with the AP-1 (A) or NF-κB (B) probes. For cold and mutated competition EMSA experiments, 100-fold molar excess of unlabeled NF-κB or AP-1 probes were included in the binding reaction. Experiments were repeated twice.