Characterization of CCR9 functional expression in secondary lymphoid organs.

(A) Cells from the sources indicated were stained with anti-CD8, anti-B220, or anti-CD4 and the biotinylated rabbit antimouse CCR9 pAb K629, and gated CD8⁺, B220⁺ and CD4⁺ cells were analyzed for CCR9 expression (bold line). Control stainings with PBSst are shown (dotted line). (B) Flow cytometry analysis of lymphocytes migrated in response to 300 nM CCL25 or to control buffer. Lymphocytes in the lower chambers of Transwell inserts were counted and stained with anti-CD4 or anti-B220 and anti-CD8 pAbs. The percentage of cells in each quadrant is shown (upper right). Representative results are shown from 3 independent experiments performed.