Age-related effects on CCR9 expression and function in thymocytes.

(A) Total thymocytes from mice of the indicated age were stained with the biotinylated rabbit antimouse CCR9 pAb K629 followed by streptavidin-RPE. A control staining with PBSst is shown. (B) The migration response to 300 nM CCL25 by total thymocytes from mice of the indicated age was assayed in Transwell inserts, and a migration index was established as in Figure 3 (▪). The chemotactic component of this migration was estimated by placing CCL25 in the upper and lower chambers of the Transwell (■). Results are representative of 3 independent experiments performed.