TCR diversity of recipient or donor T cells.

Comparative analysis of TCR-Vβ CDR3 heterogeneity length in (A) CD8-depleted (CD8⁻) or (B) CD8-enriched (CD8⁺) lymphocyte suspensions derived 72 months after BMT from theWHN^−/− recipient and theWHN^+/− donor environment. RT-PCR was performed on RNA isolated from purified T-cell subsets. PCR products were separated on DNA sequencing polyacrylamide gel by using an automated ABI PRISM 377 apparatus (PerkinElmer). Band intensity was evaluated with the Gene Scan software. Size markers are indicated. TCR-Vβ families showing fewer than 5 major peaks were considered altered.