Inhibition of VEGF binding to Flt-1 by mAb KM1732 and flow cytometric analysis of HUVEC with mAbs.

(A) Inhibition of specific ¹²⁵I-VEGF binding to Flt-1 7N or KDR 7N-Fc by anti–Flt-1 mAb, KM1732. Experimental details are given in “Materials and methods.” The profiles of KM1732 (○) and control KM231 (▴) are shown. Arrowhead (dashed line), inhibitor 0 μg/mL. (B) Flow cytometric analysis of HUVEC, binding with antihuman Flt-1 mAbs, KM1730 and KM1732, or antihuman KDR mAb, KM1992. HUVECs were stained with KM1730, KM1732, KM1992, or control mouse IgG1 mAb and analyzed by flow cytometry.