American Society of Hematology

Fig. 5.

$Fig. 5. CD34+Flt-1− cells in cord blood differentiate to CD97+Flt-1+macrophagelike cells after long-term culture. / (A) Flow cytometric analysis of human cord blood mononuclear cells. The L-fraction was further analyzed by double staining with CD34 and CD97 (B), with CD34 and Flt-1 mAbs (C), or with CD34 and CD38 mAbs (D). After a 2-week culture with a cytokine mixture (see “Materials and methods”), CD34+CD38+ cells were again analyzed by flow cytometry (E). The M-fraction in panel E was further analyzed by double staining with CD34 and CD97 (F), or with CD34 and Flt-1 mAbs (G). FS indicates forward scatter; SSC, side scatter.$

CD34⁺Flt-1⁻ cells in cord blood differentiate to CD97⁺Flt-1⁺macrophagelike cells after long-term culture.

(A) Flow cytometric analysis of human cord blood mononuclear cells. The L-fraction was further analyzed by double staining with CD34 and CD97 (B), with CD34 and Flt-1 mAbs (C), or with CD34 and CD38 mAbs (D). After a 2-week culture with a cytokine mixture (see “Materials and methods”), CD34⁺CD38⁺ cells were again analyzed by flow cytometry (E). The M-fraction in panel E was further analyzed by double staining with CD34 and CD97 (F), or with CD34 and Flt-1 mAbs (G). FS indicates forward scatter; SSC, side scatter.

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