Analysis of G-CSF receptor in lymphoblasts of the patient.

FACS analysis of the lymphoblasts of the patient. The main population of analyzed cells demonstrated a low-intensity staining for CD19 (B). Minor populations corresponding to nonleukemic T or B cells consisted of CD3⁺ cells (A) and CD19^high cells (B), respectively. Staining of the cells with anti-CD114 (anti–G-CSF receptor) revealed a specific binding on about 80% of the cells (C). Two-color analysis using FITC-labeled G-CSF and CD19-PE revealed a specific binding of FITC–G-CSF on CD19^low cells but not on CD19^high cells, indicating that the leukemic blast cells of this patient are able to bind G-CSF (D). Schematic structure of the cytoplasmic domain of the G-CSF receptor (G-CSFR) mRNA (E). The nucleotide positions given below indicate the point mutations detected in the patient reported here.