Fig. 2.
Fig. 2. FVIII and vWF interaction in plasma from a patient with hemophilia A. / (A) Sepharose CL-4B gel-filtration pattern of plasma from patient 2. Normal plasma (500 μL, left panel) or plasma from patient 2 (right panel) was applied to a Sepharose CL-4B column and eluted with TBS. FVIII:Ag (○) and vWF:Ag (●) were measured in each fraction. (B) Inhibitory effect of autoantibody from patient 2 on FVIII binding to vWF. Serial dilutions of autoantibody F(ab′)2 or immune-depleted F(ab′)2 were incubated with FVIII (1 nM) for 2 hours at 37°C before being added to immobilized vWF (40 nM). Bound FVIII was detected by using peroxidase-conjugated mAb JR8. F(ab′)2, ○; immune-depleted F(ab′)2, ●.

FVIII and vWF interaction in plasma from a patient with hemophilia A.

(A) Sepharose CL-4B gel-filtration pattern of plasma from patient 2. Normal plasma (500 μL, left panel) or plasma from patient 2 (right panel) was applied to a Sepharose CL-4B column and eluted with TBS. FVIII:Ag (○) and vWF:Ag (●) were measured in each fraction. (B) Inhibitory effect of autoantibody from patient 2 on FVIII binding to vWF. Serial dilutions of autoantibody F(ab′)2 or immune-depleted F(ab′)2 were incubated with FVIII (1 nM) for 2 hours at 37°C before being added to immobilized vWF (40 nM). Bound FVIII was detected by using peroxidase-conjugated mAb JR8. F(ab′)2, ○; immune-depleted F(ab′)2, ●.

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