Fig. 3.
![Fig. 3. IEF of secreted TC. / Samples analyzed were collected after 3 independent Sephacryl S300 HR gel filtrations, and the IEF was performed in a 110 mL LKB column using a 5%-to-50% sucrose gradient in distilled water or 5M urea. The ampholine's pH ranged from 5 to 7. (Top) IEF of TC secreted by HT-29 cells transfected with a plasmid-containing TC cDNA expressed in Caco-2 cells, in native (A) and denaturing conditions (B). (Bottom) IEF in denaturing conditions of plasma TC from healthy Caucasians, homozygotes 259-P (C), homozygotes 259-R (D), and heterozygotes 259-R/P (E). The dotted lines represent TC saturated with [57Co]cbl and the continuous lines the pH values.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/97/4/10.1182_blood.v97.4.1092/5/h80410678003.jpeg?Expires=1735539727&Signature=LRFoPnV1c6~tV2weNwSp0BAcxHd6pEoYvIv-GNS9wirXNBwVmC10SBCbIMCbVq1wNeJ7xurlnLyfJh2P2MwgU0-m-uHfqe5F2e6rfp7u8Yw6v8YiDX~RHDsdLgdckKoqJvQpqxd2A8eGYXoBNImZXrtFXdU6PgEIld29dNll-9rUA9XIumDrqYRV9KIWLLPWq74VGFfGoK7ubYTb-rDO34Epl0c3IFPX-qsOsZ5dgFEGmj6P-R7yZYwW5RzOagOHtja8vvL6IIdNfl85XLf3Pp8xwFXyxsPw3EYb1j4ORIPLI~R3lGP9bq2o~MoeYqiGsJ0zexA-shbLEnhbFvlfqw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
IEF of secreted TC.
Samples analyzed were collected after 3 independent Sephacryl S300 HR gel filtrations, and the IEF was performed in a 110 mL LKB column using a 5%-to-50% sucrose gradient in distilled water or 5M urea. The ampholine's pH ranged from 5 to 7. (Top) IEF of TC secreted by HT-29 cells transfected with a plasmid-containing TC cDNA expressed in Caco-2 cells, in native (A) and denaturing conditions (B). (Bottom) IEF in denaturing conditions of plasma TC from healthy Caucasians, homozygotes 259-P (C), homozygotes 259-R (D), and heterozygotes 259-R/P (E). The dotted lines represent TC saturated with [57Co]cbl and the continuous lines the pH values.
