The factor X GATA site binds GATA-4 from liver extracts.

(A) F.X wt GATA probe was incubated with 10 μg human liver nuclear extracts in the presence of 2 μg antibodies against GATA-4 (αG4) or GATA-2 (αG2). (B) Approximately 100 μg extracts from liver, HepG2, and human erythroleukemia (HEL) cells were analyzed on Western blots using antibodies (Ab) against GATA-4 and Sp1. HepG2 cells contain much less GATA-4 when compared to the liver. Control Western blot indicates that liver and HepG2 cells contain similar amounts of Sp1 in quality and quantity. HEL cell extracts serve as negative control because they do not contain GATA-4.