Constitutive expression of SOCS mRNA in MF tumor cell lines.

(A) Total cellular RNA was isolated from unstimulated cells and subjected to RT-PCR with the indicated SOCS primers. As internal standard primers specific for TBP were used. (B) Band intensities from the gel in panel A were quantitated using a phosphor imager and SOCS expression was calculated relative to TBP expression. (C) Relative SOCS expression calculated from 3 independent experiments (mean + range).