High expression of SOCS-3 mRNA in SeAx 4405 cells.

(A) Total cellular RNA was isolated from unstimulated cells and subjected to RT-PCR with SOCS-3 and TBP-specific primers. The RT-negative sample was treated as the other samples except that RT was not added in the cDNA synthesis. The RNA samples used in lanes 1 and 3, respectively, were from 2 independent experiments. (B) Band intensities from the gel in panel A were quantitated and SOCS-3 expression was calculated relative to TBP expression.