Decreased SOCS-3 mRNA expression in MyLa 2000 cells stably transfected with dominant negative STAT3.

Total cellular RNA from 3 different ST3D cell lines (ST3D1, ST3D4, and ST3D14) and 3 different ST3WT cell lines (ST3WT6.2, ST3WT6.8, and ST3WT6.12) was subjected to RT-PCR with CIS (A), SOCS-1 (B), SOCS-2 (C), or SOCS-3 (D) specific primers. TBP-specific primers were used as internal standard. (E-H) Band intensities from the gels in panels A to D plus from an additional and independent experiment were quantitated and SOCS expression was calculated relative to TBP expression (shown values are mean + range).