Exogenous gene expression in mice treated with primary B cells infected with MPL-PURO vector virus-expressing PLP.

(A) RT-PCR was performed on total RNA extracted from the spleens of mice treated with MPL-PURO–infected B cells (lanes 2-7) and mice treated with B cells infected with the control vector MML-PURO (lanes 8 and 9). An untreated mouse was used as negative control (lane 10), and a MPL-PURO–infected WEHI 231 B-cell line was used as positive control (lane 1). PA indicates paralysis. (B) RT-PCR with the use of MML-specific primers was performed on total RNA extracted from the spleens of mice treated with MML-PURO–infected B cells, lanes 1 and 2. RNA extracted from the spleen of an untreated mouse (lane 3) and from MPL-PURO–infected WEHI 231 B cells (lane 4) were used as negative controls. For all samples, amplification of the housekeeping geneGAPDH was performed to ensure that equal levels of total RNA were used.