Fig. 1.
Phenotypic analysis of splenic lymphocytes following T-cell–depleted allogeneic BMT.
Lethally irradiated C3H/HeJ mice each received a transplantation with ≥ 2 × 106 T-cell–depleted allogenic BM cells from CD2F1 mice a number of weeks before phenotypic analysis. Splenocytes were reacted with FITC-conjugated anti-CD3, anti-CD45R/B220, anti-CD4, or anti-CD8 mAbs or, for double-staining, with FITC-conjugated anti-CD4 mAb followed by PE-conjugated anti-CD44 or anti–L-selectin (clone MEL-14) mAb. Cells were analyzed with a FACScan flow cytofluorometer, and the data were expressed as percentage of positive cells over total cells analyzed.
