Cytokines using the common γ receptor chain up-regulate expression of mFL and sFL in T lymphocytes.

T cells were purified from normal peripheral blood (see “Patients, materials, and methods”) and cultured for 72 hours in the presence of indicated cytokines. (A-D) Expression of mFL was analyzed by FACS. (E) Release of sFL to culture supernatants was measured with ELISA. (A) Expression of mFL by resting T cells (ex vivo) and in response to IL-2 (15 ng/mL), IL-7 (15 ng/mL), or IL-7 (15 ng/mL) plus CHX (10 μg/mL). Dotted line indicates staining with isotype-matched control mAb. (B) Expression of mFL (mean ± SD) in response to increasing concentrations of IL-2, IL-4, IL-7, and IL-15. (C) Expression of mFL in response to IL-2 and IL-7 combined at indicated concentrations. Results are shown as percent ± SEM of an increase above the baseline. (D) Expression of mFL in response to IFN-γ (500 U/mL), TNF-α (100 ng/mL), and without cytokine addition (no cytokine). Dotted line indicates staining with isotype-matched control mAb. (E) Release of sFL without cytokine addition (no cytokine), and in response to the indicated cytokines; mean ± SD is shown. Concentration of cytokines, as above; IL-6 and GM-CSF were used at 100 ng/mL.