CsA inhibits expression of mFL and sFL in T lymphocytes.

(A) FACS analysis of mFL in cells cultured with IL-7 (15 ng/mL) for 72 hours in the absence or presence of CsA (5 μg/mL), ascomycin (Asc; 1 μM), or PSC833 (10 μM), as shown. Dotted line indicates staining with isotype-matched control mAb. (B) Effect of increasing concentrations of CsA on sFL release in response to IL-2 or IL-7 (15 ng/mL each). Results of ELISA measurements are shown as percent ± SEM of sFL induced by IL-2 or IL-7 in the absence of CsA after subtracting the amount expressed spontaneously.