Fig. 2.
Fig. 2. Humoral anti-idiotypic response after vaccination with 38C13 Id adenoviruses versus Id-KLH protein plus adjuvant. / (A) Groups of 10 mice were given a single injection of 38C13 Id-encoding adenovirus containing either syngeneic murine constant regions or xenogeneic human constant regions (Ad-IdhuCR) or 2 biweekly vaccinations of 38C13 Id-KLH protein along with SAF1 or QS21 adjuvant. Three weeks later, sera were collected and analyzed for anti-38C13 Id IgG antibodies by ELISA. Bars represent mean determinations in 10 animals, with error bars showing the mean ± SD. Results are representative of 3 individual experiments. (B) Isotype profiles of anti-Id antibodies elicited by adenovirus versus Id-KLH protein vaccines. Sera collected as above were pooled, and 38C13 Id-specific antibodies were quantitated by ELISA using γ1- or γ2a-specific detectors. Results are representative of 2 experiments.

Humoral anti-idiotypic response after vaccination with 38C13 Id adenoviruses versus Id-KLH protein plus adjuvant.

(A) Groups of 10 mice were given a single injection of 38C13 Id-encoding adenovirus containing either syngeneic murine constant regions or xenogeneic human constant regions (Ad-IdhuCR) or 2 biweekly vaccinations of 38C13 Id-KLH protein along with SAF1 or QS21 adjuvant. Three weeks later, sera were collected and analyzed for anti-38C13 Id IgG antibodies by ELISA. Bars represent mean determinations in 10 animals, with error bars showing the mean ± SD. Results are representative of 3 individual experiments. (B) Isotype profiles of anti-Id antibodies elicited by adenovirus versus Id-KLH protein vaccines. Sera collected as above were pooled, and 38C13 Id-specific antibodies were quantitated by ELISA using γ1- or γ2a-specific detectors. Results are representative of 2 experiments.

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