Fig. 2.
Fig. 2. PP2A-selective inhibitors block BAD dephosphorylation in vivo. / (A) FL5.12BCL-XL/BAD cells were treated with PP1 and PP2A inhibitors calyculin A, fostriecin, and tautomycin in the presence of IL-3 for 3 hours. BAD was analyzed by Western blotting as in Figure1. (B) Cells were treated with an activator (2 μM A23187) or an inhibitor (cyclosporin A or FK506) of PP2B (calcineurin) and analyzed for BAD by Western blotting.

PP2A-selective inhibitors block BAD dephosphorylation in vivo.

(A) FL5.12BCL-XL/BAD cells were treated with PP1 and PP2A inhibitors calyculin A, fostriecin, and tautomycin in the presence of IL-3 for 3 hours. BAD was analyzed by Western blotting as in Figure1. (B) Cells were treated with an activator (2 μM A23187) or an inhibitor (cyclosporin A or FK506) of PP2B (calcineurin) and analyzed for BAD by Western blotting.

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