Serine/threonine phosphatase inhibitor calyculin A rescues FL5.12 cells expressing BAD from apoptosis after IL-3 withdrawal.

Cells expressing wild-type BAD (A) or the phosphorylation-defective mutant BAD (B) were treated with 5 nM calyculin A (⋄, ▵) or vehicle alone (■, ○) for 4 hours. Cells were then cultured in the presence (⋄, ■) or absence (▵, ○) of IL-3. Viability was assayed by trypan blue exclusion at the indicated times after IL-3 withdrawal. Data are mean ± SD of triplicate assays and are representative of 3 experiments.