Increased circulating IL-3, but not GM-CSF, in mice with

BCR/ABL-induced CML-like disease. (A) Total mean plasma IL-3 levels for mice transplanted with nontransduced marrow (control BMT), with marrow transduced with MSCVneo virus (MSCVneo), or for mice with BCR/ABL-induced CML-like leukemia. Bars indicate SE. The number of mice analyzed were: control BMT = 6, MSCVneo BMT = 5, BCR/ABLcombined = 14, BCR/ABL in MSCV-IRES/GFP = 6, BCR/ABL in MSCVneo = 8. The difference between control BMT and mice with leukemia induced by BCR/ABL-MSCV-IRES/GFP, or by the 2BCR/ABL vectors combined, was significant (ttest, P = .02), whereas the difference between controls and mice with BCR/ABL-MSCVneo-induced leukemia was of borderline significance (P = .06). (B) Total mean plasma GM-CSF levels for the same control groups as in panel A, and for all mice with BCR/ABL-induced leukemia. There was no significant difference in GM-CSF levels between the groups.